Ricinine Nitrilase
نویسندگان
چکیده
منابع مشابه
Catalysis in the nitrilase superfamily.
Recently, we defined the nitrilase superfamily as consisting of 12 families of amidases, N-acyltransferases and presumptive amidases, in addition to the family of plant and bacterial nitrilases for which the superfamily was named. A novel Glu-Lys-Cys catalytic triad, found at the crystallographically defined Nit active site of worm NitFhit, was postulated to constitute the catalytic residues fo...
متن کاملBiosynthesis of the pyridine ring of ricinine.
The most widely distributed substituted pyridine compounds in living systems are the pyridine nucleotides. The nicotinamide moiety (Fig. 1, I) of these compounds arises from the indole nucleus of tryptophan in animals, (1, 2) and Neurospora (3, 4), but not in higher plants (5-7) and certain bacteria (8). Pyridine compounds which might be used to study the biosynthesis of the pyridine nucleus ar...
متن کاملA nitrilase gene cyc705 from Arthrobacter aures-
cens CYC705 for synthesis of iminodiacetic acid (IDA) was cloned. This gene contained a 930 bp ORF, which encoded a polypeptide of 310 amino acids. A recombinant Escherichia coli BL21(DE3)/ pET28a-cyc705 was constructed to achieve the heterologous expression of cyc705. This recombinant nitrilase was purified to homogeneity with a molecular weight of 36.7 kDa on SDS-PAGE and mass spectrometry, a...
متن کاملSerial ricinine levels in serum and urine after ricin intoxication.
Ricinine is an alkaloid present in the castor bean plant (Ricinus communis) that can be used as a biomarker for ricin poisoning. Serial ricinine levels are reported in the serum and urine of a patient suffering from intentional ricin intoxication. The patient was brought to the hospital 4 h after injection and oral intake of a castor bean extract, but died 38 h later, despite intensive medical ...
متن کاملExploring nitrilase sequence space for enantioselective catalysis.
Nitrilases are important in the biosphere as participants in synthesis and degradation pathways for naturally occurring, as well as xenobiotically derived, nitriles. Because of their inherent enantioselectivity, nitrilases are also attractive as mild, selective catalysts for setting chiral centers in fine chemical synthesis. Unfortunately, <20 nitrilases have been reported in the scientific and...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1964
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(18)91167-1